%0 Journal Article
%T Synthesis of Total sFat-1 Gene by PCR-Restrict Enzyme Ligation Method<br>应用PCR-酶切连接法合成全长sFat1基因
%A ZHU Gui-Ming
%A CHEN Hong
%A LU Jian-Shen
%A ZHOU Yan-Rong
%A WU Xiao-Jie
%A CHEN Hong-Xing
%A DENG Ji-Xian
%A <br>朱贵明
%A 陈 宏
%A 卢建申
%A 周艳荣
%A 吴晓洁
%A 陈红星
%A 邓继先
%J 遗传
%D 2006
%I 
%X Gene synthesis is very important in life science research, and it becomes a technique in common use. It is difficult for long gene synthesis, because the mismatches and mutations of DNA sequence in nucleotide fragments assembling. This study established a new method for long gene synthesis, which was referred to as PCR-restrict enzyme ligation method. With this method, a omega-3 fatty acid desaturase gene, sFat-1, from Caenorhabditis briggssae, was successfully assembled from 27 synthesized nucleotide fragments (60 ~ 68 bp for each fragment ) following 3 rounds of PCR (7 reactions) and 2 rounds of restrict enzyme ligation (3 reactions). This shows that the PCR-restrict enzyme ligation method is an effective method for long gene synthesis.
%K 基因合成
%K 聚合酶链式反应
%K ω-3脂肪酸去饱和酶基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=34AAAEA07DBC9235&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=9CF7A0430CBB2DFD&sid=D60A23E3DA48A028&eid=A91C28383511878F&journal_id=0253-9772&journal_name=遗传&referenced_num=5&reference_num=11