%0 Journal Article
%T Accelerated Somatic Cell Senescence and Changes in p16INK4a Expression after Exogenous DNA Transfection<br>外源基因转染导致小鼠体细胞过快衰老 及p16INK4a表达水平变化
%A SHEN Wei
%A LI Lan
%A WU Xiao-Jie
%A ZHOU Yan-Rong
%A PAN Qing-Jie
%A CHEN Hong
%A DENG Ji-Xian
%A <br>沈 伟
%A 李 兰
%A 吴晓洁
%A 周艳荣
%A 潘庆杰
%A 陈 宏
%A 邓继先
%J 遗传
%D 2006
%I 
%X During the transfection of mouse embryonic fibroblasts (MEF), we found these cells became senescent, appearing enlarged with hollow cytoplasm and multinucleated. The telomere lengths of these senescent MEFs were significantly shorter than primary untransfected MEFs. In senescent MEF cells, DNA methylation of p16INK4a 5'-regulatory region showed gradual reduction as cells aged. RT-PCR and Northern blot demonstrated that the expression of p16INK4a in transfected senescent cells was 12 - 16 times more than primary cells. The senescent transfected MEF cells as donors of nuclei could support the early development of cloned embryos after nuclear transfer.
%K mouse
%K exogenous gene transfection
%K cell senescence
%K telomere
%K p16INK4a gene<br>小鼠
%K 转基因
%K 细胞衰老
%K 端区
%K p16INK4a基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=D31E8DE4EE9D7F20&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=708DD6B15D2464E8&sid=59754DBA76F6BBAA&eid=77586D59F496BF38&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=18