%0 Journal Article
%T Construction of A New Vector for Direct Cloning of PCR Products<br>可直接克隆PCR产物的克隆载体的构建 Construction of A New Vector for Direct Cloning of PCR Products
%A HU Xue-jun
%A LI Jing-quan
%A YUAN Xiao-dong
%A XU Hong-mei
%A ZHAO Dong-li
%A <br>胡学军
%A 李晶泉
%A 袁晓东
%A 徐红梅
%A 赵东利HU Xue-jun
%A LI Jing-quan
%A YUAN Xiao-dong
%A XU Hong-mei
%A ZHAO Dong-li
%J 遗传
%D 2002
%I 
%X A new method for construction of a cloning vector (T-vector) for direct ligation with PCR products was described. The T-vector derived from pUC118 in which the unique restriction site of Eam1105 I in the region of Amp(r) gene was deleted and an artificial DNA fragment flanking two Eam1105 I was introduced at the site of BamHI. The modified vector was named as pUC118E. A T-vector with 3' over hang end of a single T can be obtained via digesting of pUC118E with Eam1105I. PCR products can be easily cloned with this T-vector.
%K T-vector
%K pUC118
%K PCR products
%K Eam1105 I<br>T－载体
%K pUC118
%K PCR产物
%K 限制酶Eam1105I
%K 构建
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=B0B1DA21E639A26C&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=6425DAE0271BB751&eid=4609832E4B5C797B&journal_id=0253-9772&journal_name=遗传&referenced_num=2&reference_num=3