%0 Journal Article
%T Construction of a Fused fpg Gene by Using TP-PCR Method<br>PCR一步法构建融合蛋白基因fpgConstruction of a Fused fpg Gene by Using TP-PCR Method
%A LIU He~
%A CHEN Ying-Xu~
%A ZHANG Wen-Bo~
%A JIN Yong-Feng~
%A <br>刘 和
%A 陈英旭
%A 张文波
%A 金勇丰LIU He
%A CHEN Ying-xu
%A ZHANG Wen-bo
%A JIN Yong-feng
%J 遗传
%D 2004
%I 
%X TP-PCR,a method developed for fusion gene construction without the use of endonuclease and ligase, was performed to construct a fused fpg gene. The TP-PCR reaction system contained three primers and two templates and resulting PCR product, fused fpg gene, consisted of three sections: pheB gene, which was responsible for catechol 2,3-dioxygenase, gfp gene for GFP protein and the intermediate ligation segment which was designed for the correct expression of the fusion gene. The result in this paper showed that the TP-PCR method is one of rapid and convenient methods for fused gene construction.
%K TP-PCR
%K Catechol 2
%K 3-dioxygenase
%K green fluorescent protein
%K fusion gene<br>PCR一步法
%K 邻苯二酚双加氧酶
%K 绿色荧光蛋白
%K 融合基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=08F2F841A906893A&yid=D0E58B75BFD8E51C&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=20C3B205768D55E0&eid=652E7E360EBE3082&journal_id=0253-9772&journal_name=遗传&referenced_num=7&reference_num=7