%0 Journal Article
%T Cloning of ZFY /ZFX Gene of the Tufted Deer and Identification of its Sex<br>毛冠鹿ZFY、ZFX基因片段的克隆与性别鉴定 Cloning of ZFY /ZFX Gene of the Tufted Deer and identification of its sex
%A JIANG Hua-Yun~
%A CAO Xiang-Rong~
%A ZHANG Xi-Ran~
%A HU Jun~
%A XU Chun-Mao~
%A <br>蒋华云
%A 曹祥荣
%A 张锡然
%A 胡 均
%A 徐春茂JIANG Hua-Yun
%A CAO Xiang-Rong
%A ZHANG Xi-Ran
%A HU Jun
%A XU Chun-Mao
%J 遗传
%D 2004
%I 
%X According to the human sex differentiation related ZFY and ZFX genes, a pair of primers were designed , and fragments were amplified from the genomic DNA of male or female tufted deer. Subsequently the amplified fragments were cloned into the vector pMD18T and were sequenced.It is found that the sequences of ZFY gene and ZFX gene have 91% homology. Based on the different nucleotides, restriction site of Ava II was found to be specific to ZFX gene. The results show that the combination of PCR with Ava II digestion is a simple and sensitive way to identify the tufted deer sex.
%K Elaphodus cephalophus
%K ZFY
%K ZFX
%K sexual identification<br>毛冠鹿(Elaphoduscephalophus)
%K ZFY
%K ZFX
%K 性别鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=3FD40CE51A32F1D4&yid=D0E58B75BFD8E51C&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=BC88D6B0750E09D1&eid=3FC4D669D19FF0C6&journal_id=0253-9772&journal_name=遗传&referenced_num=7&reference_num=7