%0 Journal Article
%T The Research on the Relationship Between the Polymorphism of T105A Locus in MC1R Gene and Coat Color in Dogs<br>犬MC1R基因T105A基因座多态性及 与毛色性状相关性的研究The Research on the Relationship Between the Polymorphism of T105A Locus in MC1R Gene and Coat Color in Dogs
%A GUO Duo~
%A SU Yu-Hong~
%A BA Cai-Feng~
%A ZHU Bao-Qin~
%A ZHANG Yi-Bo~
%A LI Ning~
%A CHEN Qing-Hua~
%A GU Xue-Jing~
%A <br>郭 多
%A 苏玉虹
%A 巴彩凤
%A 朱宝芹
%A 张轶博
%A 李 宁
%A 陈清华GUO Duo
%A SU Yu-Hong
%A BA Cai-Feng
%A ZHU Bao-Qin
%A ZHANG Yi-Bo
%A LI Ning
%A CHEN Qing-Hua
%J 遗传
%D 2004
%I 
%X In order to detect the polymorphism of T105A in MC1R gene in dogs and to analyze the relationship between the genetic polymorphisms and phenotypes of dog coat color, the blood samples of 111 cross-breed dogs were taken and their genomic DNAs were extracted. The phenotypes of dog coat color were recorded. The T105A locus of MC1R gene in the canine was detected through the technology of PCR-RFLP. Furthermore, the polymorphic fragments at T105A were sequenced. The relationships between the polymorphism of T105A and coat color trait were analyzed by the statistical methods of bivarate correlation analysis. By the method of PCR-RFLP, the T105A polymorphism was found with two alleles A and B and three genotypes AA, AB and BB.The frequencies of two alleles were 72.97% and 27.03%, respectively. The heterozygosity of T105A locus was 0.39. The frequencies of three genotypes were 55.86%, 34.23% and 9.91%, respectively. According to the results of sequencing, one base change from G to A at the position 105 was found at T105A locus and it altered amino acid at the position 105 from alanine to threonine. According to the statistical analysis, no significant association between the polymorphism of MC1R gene and the coat color was found and the result may be due to the differences of genetic background. Further research on MC1R gene should be done in pure breed dogs.
%K canine
%K MC1R gene
%K T105A locus
%K PCR-RFLP
%K coat color<br>犬
%K MC1R基因
%K T105A基因座
%K PCRRFLP
%K 毛色
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=A74CFBD3EF021CDB&yid=D0E58B75BFD8E51C&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=366B1248A15658C5&eid=3389C664025A98F9&journal_id=0253-9772&journal_name=遗传&referenced_num=4&reference_num=14