%0 Journal Article
%T 一种新的cDNA 末端快速扩增获取全长cDNA的方法 A Novel Method of Rapid Amplification of cDNA Ends for Obtaining the Full-Length cDNA
%A 邱为民
%A 张思仲
%A 武辉
%A 张戈
%A 肖翠英QIU Wei-min
%A ZHIANG Si-zhong
%A WU Hui
%A ZHANG Ge
%A XIAO Cui-ying
%J 遗传
%D 2001
%I 
%X 为克隆精子发生相关基因的全长cDNA,根据mRNA差异显示获得的ESTs设计引物,利用一种新的cDNA末端快速扩增方法(SMARTRACE)扩增该EST的5′末端,并进行克隆测序,与mRNA差异显示获得ESTs拼接后,获得了三个新的全长cDNA.结果表明,SMARTRACE是一种简便、有效的克隆cDNA5′末端未知序列的技术. Abstract:To clone the full-length cDNAs of genes related to spermatogenesis,ESTs obtained by mRNA differential display were used to design gene-specific primer.Then SMART RACE was performed to obtain the 5′ region of these ESTs.After cloning,sequencing and splicing with ESTs obtained by mRNA differential display,three full-length cDNAs were obtained.The results indicate that SMART RACE is a simple and an effective technique for cloning 5′-end unknown sequence of gene.
%K cDNA
%K 末端快速扩增
%K 精子发生相关基因
%K Key
%K words
%K SMART
%K RACE
%K spermatogenesis-related
%K gene
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=F88E7E5FDD24ECC39B01F2E96BA516AF&yid=14E7EF987E4155E6&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=03436AC72A659ACA&eid=283B38DAD0D068F3&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=0