%0 Journal Article
%T Rapid Construction of Full-length MnSOD cDNA of Chickens by One-step 3′RACE<br>一步3’RACE快速构建鸡MnSOD全长cDNA克隆Rapid Construction of Full-length MnSOD cDNA of Chickens by One-step 3’RACE
%A BU You-Quan
%A LUO Xu-Gang
%A LIU Bin
%A LI Su-Fen
%A <br>卜友泉
%A 罗绪刚
%A 刘 彬
%A 李素芬BU You-Quan
%A LUO Xu-Gang
%A LIU Bin
%A LI Su-Fen
%J 遗传
%D 2004
%I 
%X RACE (rapid amplification of cDNA ends) is a popular technique to rapidly obtain the full-length cDNA. After obtaining the 3' cDNA and 5' cDNA fragments with a overlapped region by 3' RACE and 5' RACE, the full-length cDNA could be generated by end-to-end PCR or subcloning. In this study, 3' RACE combined with touch-down PCR was successfully used for the rapid construction of full-length MnSOD cDNA of chickens. Compared with the conventional end-to-end PCR or subcloning, this method, called one-step 3' RACE, is fast, economical and highly specific. It especially fits the rapid construction of full-length cDNA by RACE method.
%K RACE
%K touch-down PCR
%K MnSOD
%K Cdna<br>RACE
%K 触减PCR
%K MnSOD
%K cDNA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=14FF35425E8D21B9&yid=D0E58B75BFD8E51C&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=89389F643CA3F778&eid=68FF5306A8F9C315&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=4