%0 Journal Article %T Analysis of the chromosomes of S. bicolor × S. propinquum and S. dochna × S. propinquum by FISH
FISH分析栽培高粱×拟高粱、甜高粱×拟高粱的染色体行为 %A XIE Li %A HAN Yong-Hua %A LI Dong-Yu %A ZENG Yan-Hua %A
谢莉 %A 韩永华 %A 李冬郁 %A 曾艳华 %J 遗传 %D 2009 %I %X By means of fluorescence in situ hybridization (FISH), we located and analyzed the sites of the 45S rDNA on the F1 hybrids of S. bicolor × S. propinquum and S. dochna×S.propinquum. Two signals of 45S rDNA were mapped on the mitotic metaphase chromosomes in the F1 hybrids, respectively, and one signal was present on a bivalent during meiotic of synaptene, diakinesis, and metaphase I. We inferred that the two mitotic chromosomes carrying 45S rDNA were homolo-gous pairs. Considering the signals of 45S rDNA location during meiotic process, chromosome pairing of these two F1 hy-brids was normal with an average pairing configuration of 2n=2x=20 (10 II). Our results indicated that 45S rDNA could provide a landmark for identification of individual chromosome during meiosis indirectly. %K 45S rDNA
栽培高梁 %K 甜高梁 %K 拟高梁 %K 荧光原位杂交 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=D89E8EA37FD43AE8AB144F2A0CD7D322&yid=DE12191FBD62783C&vid=4AD960B5AD2D111A&iid=E158A972A605785F&sid=5335AD3CFE6E14EA&eid=F10601728A1E9BEA&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=16