%0 Journal Article
%T The Simple Gain of Templates of Rice Genomes DNA for PCR<br>水稻PCR扩增模板的快速制备 The Simple Gain of Templates of Rice Genomes DNA for PCR
%A Xian-Chun Sang
%A Guang-Hua He
%A Yi Zhang
%A Zheng-Lin Yang
%A Yan Pei
%A <br>桑贤春
%A 何光华
%A 张毅
%A 杨正林
%A 裴炎
%J 遗传
%D 2003
%I 
%X The solution of alkali-treated fresh rice leaves was used directly as the templates of PCR. The amplified results were stable, reliable, and had no difference compared with that amplified with rice total DNA extracted by common method. The stable results can still be obtained based on the templates kept at 25 degrees for tow weeks, at 4 degrees for three weeks, at -20 degrees for over four months. With this technique, less material and only common reagent are required, which is especially adapted to the screening of the precious transgenic rice in advance and large-scale PCR tests.
%K rice
%K genomic DNA
%K PCR templates
%K methodology<br>水稻
%K 基因组DNA
%K PCR模板
%K 方法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=C7EDBF53089F4794&yid=D43C4A19B2EE3C0A&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=703F3C1B6594BA64&eid=E008F9AD6D4B96EF&journal_id=0253-9772&journal_name=遗传&referenced_num=26&reference_num=9