%0 Journal Article
%T Goat β-lactoglobulin Gene Cloning and High Expression in the Mammary Gland of Transgenic Mice<br>山羊β乳球蛋白基因的克隆及其在转基因小鼠乳汁中的高效表达 Goat β-lactoglobulin Gene Cloning and High Expression in the Mammary Gland of Transgenic Mice
%A Jian-Jun Gao
%A Jing-Bin Yan
%A Ying Huang
%A Yi-Tao Zeng
%A <br>高建军
%A 颜景斌
%A 黄英
%A 曾溢滔
%J 遗传
%D 2003
%I 
%X To clone goat beta-lactoglobulin (BLG) gene,two fragments were amplified from goat genomic DNA by LD-PCR method. The fragments were inserted in T-vectors before being spliced into the whole 7.2 kb BLG gene at a single restriction enzyme site of NarI. Consequently, the eukaryotic expression vector was constructed. All the clones were proved to be correct by restriction enzyme cutting and sequencing analysis. Six Founders (3 male symbol, 3 female symbol) of goat BLG transgenic mice were obtained by microinjection and BLG genes integration were confirmed by both PCR and Southern blot analyses. The milk was collected from two lactating female transgenic mice and goat BLG protein contents were measured with ELISA. The results showed that goat BLG protein in milk of the two mice were 23.49 mg/mL and 2.19 mg/mL, respectively.
%K 转基因小鼠
%K 乳腺表达
%K 乳汁蛋白
%K 山羊β乳球蛋白基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=56720447DC7B4BD3&yid=D43C4A19B2EE3C0A&vid=C5154311167311FE&iid=94C357A881DFC066&sid=A03A15CF5604A8B0&eid=9296A146D1D94BC4&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=17