%0 Journal Article
%T RAPD分析在绢丝昆虫亲缘关系研究中的应用Ⅱ.柞蚕品种间的遗传差异Application of RAPD Technique in Genetic Relationship of Silk Insect II. Genetic Variance in Antheraea pernyi
%A 桂慕燕
%A 左正宏
%A 王学民
%A 陈元霖GUI Mu-yan
%A ZUO Zheng-hong
%A WANG Xue-min
%A CHEN Yuan-lin
%J 遗传
%D 2001
%I 
%X 采用RAPD技术,对5个柞蚕品种的遗传差异进行比较研究.结果表明,所采用的40个随机引物中,有27个引物扩增谱带清晰且重复性较好,扩增总片段数253条,单个引物的扩增片段数在4～16之间,片段大小在0.33～3.0kb之间.不同柞蚕品种间的遗传差异较小,遗传距离(D)在0.066～0.1659之间,根据D值,由UPGMA聚类分析软件绘制了它们的分子进化树。 Abstract:Random amplified Polymorphic DNA (RAPD) was used to analyze the genetic diversity among Antheraea pernyi.The genetic variance of five Antheraea pernyi was studied.The result showed that:27 of 40 arbitrary primers could amplify clear and repeating bands.A total of 262 fragments were obtained.Each primer gave 4~16 bands and the average was 9.7.The length of the band was 0.33~3.0kb.The D value between different breeds of Antheraea pernyi was 0.066~0.1659.The D value was used to construct a dendrogram by UPGMA.
%K 柞蚕
%K RAPD
%K 品种差异
%K Key
%K words
%K Antheraea
%K pernyi
%K RAPD
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=8683D3542BEDABE136EC927A5BC409FE&yid=14E7EF987E4155E6&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=30F3EEEA29E34EE7&eid=6C62BFE34266FA92&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=0