%0 Journal Article
%T Cloning and analysis of adenine nucleotide translocase gene in Heli-coverpa armigera
棉铃虫腺苷酸转移酶基因的克隆与分析
%A NIU Bao-Long
%A WENG Hong-Biao
%A HE Li-Hua
%A SHEN Wei-Feng
%A QI Xiao-Peng
%A LIU Yan
%A MENG Zhi-Qi
%A
牛宝龙
%A 翁宏飚
%A 何丽华
%A 沈卫锋
%A 齐晓朋
%A 刘岩
%A 孟智启
%J 遗传
%D 2008
%I
%X A cDNA clone encoding the ADP/ATP translocase in Helicoverpa armigera has been identified by RT-PCR, 5'and 3'RACE methods. Sequence analysis shows that it is 1,190 bp long and contains a single open reading frame (ORF, 133-1,033 bp) encoding a protein of 300 amino acids (GenBank submission number, AY253868). The protein has a 22 aa signal peptide on its N-terminal, which leads the protein locating onto the inner membrane of the mitochondria. It also has three conserved domains of the mitochondrial carrier protein forming a channel to exchange ATP and ADP energy molecule through the inner membrane of the mitochondria. It shows extensive similarities to the known ADP/ATP translocase poly-peptides. The ADP/ATP translocase similarity was up to 90% in the Lepidoptera.
%K 棉铃虫
%K 腺苷酸转移酶
%K 克隆
%K 功能预测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=AC4C1A006D3265FDBBA8B88B0EE712C1&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=CA4FD0336C81A37A&sid=35FC3610259C2B32&eid=7AA74D31F1FF2DCE&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=10