%0 Journal Article
%T A co-dominant molecular marker of fusarium wilt resistance gene I-2 derived from gene sequence in tomato<br>由基因序列开发番茄枯萎病抗性基因I-2的共显性分子标记
%A Shuan-Cang Yu
%A Yan-Min Zou
%A <br>于拴仓
%A 邹艳敏
%J 遗传
%D 2008
%I 
%X Sequence-specific PCR primers, I-2/5F and I-2/5R were designed according to the sequence from 3 132 bp to 3 765 bp in I-2 gene. With them a 633 bp fragment was amplified from 03F-7 with a genotype of I-2 / I-2, 693 bp fragment from Moneymaker with a genotype of i-2/ i-2, and both fragments from Tebao with heterozygous genotype I-2 / i -2. These two specific fragments were cloned and sequenced. The results from multiple sequence alignment showed that the 633bp fragment from 03F-7 was identical with the sequence from 3 132 bp to 3 765 bp in I-2 gene. Compared with the sequence of I-2 gene, there were a large number of mutations and a 60 bp fragment inserted in susceptible alleles. The PCR primer com-bination, I-2/5F and I-2/5R can be used to distinguish homozygous resistant, heterozygous and homozygous susceptible materials, and it is a functional co-dominant marker in I-2 gene selection. Moreover, this marker was employed on 16 major tomato varieties for I-2 locus genotyping, in which half of the varieties contain I-2 gene, and only one variety is homozy-gous resistant. In addition, simultaneous detection system for I-2 and Tm-22 was established by a single PCR and a Hind III digestion. It provides a powerful tool for multiple genes selection in tomato breeding program.
%K 番茄
%K 枯萎病
%K I-2基因
%K Tm-22基因
%K 分子标记
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=3AEE67CDD805BBD71CB62C507557852C&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=DF92D298D3FF1E6E&sid=BD0BCC5CD284A664&eid=796A97DD793AE4A8&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=0