%0 Journal Article
%T Case report of a rare platelet-specific antigen HPA-10bw allele found in Chinese mainland<br>中国大陆地区发现一例罕见的血小板抗原HPA-10bw等位基因报告
%A FENG Ming-Liang
%A SHEN Tong
%A HUANG Hui
%A SHEN Wei
%A WANG Jian-Lian
%A LIU Da-Zhuang
%A ZHAO Tong-Mao
%A <br>冯明亮
%A 沈彤
%A 黄慧
%A 沈伟
%A 王健莲
%A 刘达庄
%A 赵桐茂
%J 遗传
%D 2007
%I 
%X A total of 1,000 Chinese blood donors were typed for human platelet antigens (HPA) using a sequence specific primers -polymerase chain reaction (PCR-SSP) based HPA genotyping method. An individual with a rare HPA-10w(a+b+) genotype was found. In order to confirm the typing results, a fragment of HPA-10 gene was amplified by PCR and then sequenced. Sequencing data showed that a single G to A substitution at nucleotide 263 occurred, resulting in amino acid change from Arg(CGA) to Gln(CAA) at position 62 of GPa protein. The substitution generated antigenic specificity HPA-10bw. The detection of an HPA-10bw allele in the Chinese population suggests that this rare allele should be considered in platelet alloimmunization, such as neonatal alloimmune thrombocytopenia (NAIT), post-transfusion thrombocytopenic purpura (PTP) and post-transfusion refractoriness to platelets (PTR).
%K PCR-SSP<br>中国人群
%K HPA-10bw抗原
%K HPA基因分型
%K DNA测序
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=8F839044623B5BC0&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=0B39A22176CE99FB&sid=6425DAE0271BB751&eid=E114CF9BB47B65BE&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=7