%0 Journal Article
%T Function of inducible nitric oxide synthase on adaptability to hypoxia in Tibetan chicken<br>藏鸡诱导型一氧化氮合酶基因低氧适应功能分析
%A ZHANG Hao
%A CHAMBA Yangzom
%A ZHAO Chun-Jiang
%A BAO Hai-Gang
%A LING Yao
%A WU Chang-Xin
%A <br>张浩
%J 遗传
%D 2009
%I 
%X 低氧刺激诱导型一氧化氮合酶(Inducible nitric-oxide synthase,iNOS)催化产生NO,增加血流,改善组织供氧.文章采用测序和PCR-RFLP技术检测藏鸡及低地鸡iNOS基因编码区、5'侧翼区(2.0 kb片段)序列和3'侧翼序列SNP,并测定低氧和常氧孵化时鸡胚尿囊绒毛膜组织iNOS基因表达量和酶活力.结果在iNOS基因5'侧翼区发现一个与低氧适应相关的藏鸡高频率突变SNP位点(-870C→T),藏鸡该突变的等位基因T频率高于低地鸡种.藏鸡iNOS基因表达量和酶活力在低氧孵化环境中多高于矮小鸡.结果表明藏鸡群体iNOS基因的突变及其低氧表达量的增加是其适应低氧环境的重要基础.
%K SNP<br>藏鸡
%K 低氧适应
%K iNOS基因
%K 基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=F67D383DD80ABF3C3D0CD1BBDEBA4291&yid=DE12191FBD62783C&vid=4AD960B5AD2D111A&iid=E158A972A605785F&sid=8ACD9060100C26F1&eid=DA74B62FE4348759&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=18