%0 Journal Article
%T Differentiation of Bos grunniens, Bos Taurus, and Bubalus from meat products mixture based on mitochondrion 12S rRNA gene<br>基于线粒体12S rRNA基因鉴别混合牛肉及制品的牛种来源
%A CHEN Dong
%A BAI Fan
%A ZHOU Ming-Liang
%A ZHANG Xiang-Yu
%A WU Deng-Jun
%A <br>陈冬
%A 柏凡
%A 周明亮
%A 张翔宇
%A 吴登俊
%J 遗传
%D 2008
%I 
%X Mitochondrial DNA sequence is highly conserved within species. Gene 12S rRNA is able to endure degeneration and high temperature, which allows identification of feedstuff, fresh meat, processed meat, and traceability. In the present study, three unique restriction sites were detected in the fragments of mitochondrial 12S rRNA gene regions amplified with universal primer, which were able to distinguish Bos grunniens, Bos. taurus, and Bubalus in fresh meat and processed meat mixture. The fragment of yak was digested to 134 bp and 318 bp, scalper 134 bp and 318 bp, and buffalo 86 bp and 367 bp. The specific locus and digestion were verified by sequencing analysis. There was no difference between PCR amplification products from various treatments at different temperatures (i.e., 100, 120, 140, 160, and 180). However, the sig-nal was weak at 120 and above. This method is simple, fast and cheap in identification of fresh meat and processed meat.
%K 12S rRNA<br>酶切
%K 牛线粒体基因
%K 内源参照基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=BB11B2FA064F9CF92EEF0E918B288879&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=5D311CA918CA9A03&sid=82BCA4C44409DD5C&eid=512771C17A2FFC1B&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=26