%0 Journal Article
%T A Preliminary Study of the Structure Prediction and Expression of SARS-CoV Spike Protein<br>SARS冠状病毒S蛋白结构预测及表达初探
%A SUN Mei-Ling
%A PIAO Jin-hua
%A YIN Chang-Cheng
%A YANG Gong-she
%A HUANG Hua-liang
%A <br>孙美玲
%A 朴锦华
%A 尹长城
%A 杨公社
%A 黄华樑
%J 遗传
%D 2005
%I 
%X In this study, the encoding sequences of SARS-CoV spike protein were analyzed by bioinformatics methods, the structural characteristics and functions were forecasted based on available data. It suggests that the fragment of spike glycoprotein (S401~659) may be crucial for viral attachment and may be a major immunodominant epitope. Then the fragment was amplified and subcloned into expression vector pET28a( ) and pPIC9K. These two plasmids (pET28a( )-S) and pPIC9K-S were transformed to E.coli strain BL21(DE3)-star and Pichia pastoris, respectively. SDS-PAGE and Western blot analysis showed that the recombinant protein was successfully expressed. The denatured inclusion bodies were purified with Ni-NTA chelate agarose and its purity can reach 90%.
%K SARS<br>冠状病毒
%K S蛋白
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=5CF7EE97BFA0AF24&yid=2DD7160C83D0ACED&vid=DB817633AA4F79B9&iid=E158A972A605785F&sid=F8AEC975DBDD7F2F&eid=3EABEBD973E45554&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=20