%0 Journal Article
%T 植物叶片蛋白质双向电泳技术的改进与优化 Improvement in the Two-dimensional Electrophoresis of Proteins from the Leaves of Plant
%A 何瑞锋
%A 丁毅
%A 张剑锋
%A 余金洪HE Rui-feng
%A DING Yi
%A ZHANG Jian-feng
%A YU Jin-hong
%J 遗传
%D 2000
%I 
%X 通过对传统的双向电泳方法进行改进与优化,得到了一种适合于分析植物叶 片蛋白质的双向电泳新方法。用改进后的方法对水稻不同时期成熟叶片蛋白质进行双向电泳分离,结果显示其稳定性和重复性好,分辨率较高,经考马斯亮蓝染色后可分辨出300多个蛋白质(肽)点。它们的等电点和分子量主要分布于pI4.1-8.2和10-100kDa之间。本文还就实验过程中出现的一些技术问题进行了讨论。 Abstract:Base on the method of O farrell,an improved procedure for the two-dimensional gel electrophoresis of leaf proteins is presented.The two-dimensional separation of proteins from the mature leaves of different developmental stages of rice provides distinct and steady results.The detected protein(peptide)spots stained by Coomassie brilliant blue R-250 are more than 300,with isoelectric points(pI)ranging from 4.1 to 8.2 and molecular weights(MW)from 10 kDa to 100 kDa.
%K 水稻
%K 叶片蛋白质
%K 双向电泳
%K Key
%K words
%K rice
%K leaf
%K protein
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=086D3C7DF453528873452D7437BEAE48&yid=9806D0D4EAA9BED3&vid=BC12EA701C895178&iid=94C357A881DFC066&sid=85002451B65CE0D1&eid=4AD4BA66429F5627&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=0