%0 Journal Article
%T Isolation and comparative study of clones from human esophageal carcinoma cell line EC9706<br>人食管癌EC9706单克隆的分离培养及其生物学特性的研究
%A HAN Ya-Ling
%A FENG Yan-Bin
%A LUO Man-Li
%A XU Xin
%A CAI Yan
%A WANG Ming-Rong
%A <br>韩亚玲
%A 冯彦斌
%A 罗曼莉
%A 徐昕
%A 蔡岩
%A 王明荣
%J 遗传
%D 2007
%I 
%X Cell line is one of the important materials for cancer research. In the present study, single-cell clones were iso- lated from esophageal carcinoma cell line EC9706 by limited dilution method. Three sublines were obtained, namely EC1, EC2 and EC3. Cytogenetic analysis showed that EC1 was a mixed clone, and the chromosome number of sublines EC2 and EC3 were 117 and 62, respectively. There were significant differences in the growth rate, cell cycle distribution, cell mobil- ity, and colony formation ability between EC2 and EC3. The degree of malignancy was significantly higher for EC3 than for EC2. The monoclonal sublines EC2 and EC3 with different malignant potential provide useful materials for exploring the pathogenesis and gene therapy of human esophageal carcinoma, investigating multidrug resistance, and screening anti-tumor medications.
%K esophageal squamous cell carcinoma
%K cell line
%K fluorescence in situ hybridization
%K immunocytochemistry<br>食管癌
%K 细胞系
%K 荧光原位杂交
%K 免疫细胞化学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=00485C538484E5ED0ABDD1956B50B936&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=708DD6B15D2464E8&sid=09002DF587B7129E&eid=65C780F1B91D7CD5&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=7