%0 Journal Article
%T The Immune Responses of the Fusion Protein Including Two Copies of T-cell and B-cell Epitopes of Food-and-mouth Disease Virus VP1 Type O and LTB and STI Enterotoxins of Escherichia coli<br>O型口蹄疫病毒VP1 T细胞、B细胞表位双拷贝基因与大肠杆菌LTB、STI肠毒素基因融合表达产物的免疫应答
%A ZHUANG Juan
%A YOU Yong-Jin
%A CHEN Bo
%A RAO Zhong
%A PAN Jie
%A <br>庄娟
%A 尤永进
%A 陈波
%A 饶忠
%A 潘洁
%J 遗传
%D 2006
%I 
%X A nucleotides fragment 2020VP1 related to T-cell epitope (aa21-40) and B-cell epitope (aa141-160) of VP1 of Food-and-mouth disease virus (FMDV)type O was synthesized, and the recombined expression vector r2020-LTB-2020-STI was correctly constructed, which was used to express the fusion protein consisted of two copies of aa21-40aa141-160 of FMDV VP1 and LTB and STI Enterotoxins of Escherichia coli. The fusion protein with molecular weight of about 45kD was successfully expressed in E.coli BL21(DE3) RIL at high level and confirmed by SDS-PAGE. The purified fusion protein could be specifically recognized by CTB antibody. The purified protein was then used to vaccinate guinea pigs, rabbits and Balb/c mice at 6-8 week age, and immune responses were finally observed. The fusion protein could induce proliferation of spleen T cells in vaccinated guinea pigs and elicit a high level of neutralizing antibody in rabbits. It could be concluded that the fusion protein could activate FMDV-specific cellular immune-response and humoral immune-response simultaneously. At the same time, the vaccinated mice could survive challenge of Enterotoxigenic Escherichia coli C83902, and the sera of vaccinated rabbits could neutralize the STI toxin. Moreover, the fusion protein had no STI toxicity, indicating that the fusion protein had LTB and STI immunogenicities. Therefore, it can be concluded that the fusion protein could be explored as a potentially efficient FMDV and ETEC vaccine.
%K VP1
%K LTB<br>口蹄疫病毒
%K T细胞表位
%K B细胞表位
%K 肠毒素大肠杆菌
%K STⅠ
%K 免疫应答
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=BD15EA4F13FD9119&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=94C357A881DFC066&sid=2E15A588990CC690&eid=12AD09BCF4A6E651&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=13