%0 Journal Article
%T Construction and analysis of cDNA library of Yunnan Yuanjiang O. rifupogon leaf<br>元江普通野生稻叶片cDNA文库的构建及部分基因片段分析
%A SHI Dong-Yan
%A YANG Ming-Zhi
%A HUANG Xing-Qi
%A CHEN Shan-Na
%A CHENG Zai-Quan
%A <br>史冬燕
%A 杨明挚
%A 黄兴奇
%A 陈善娜
%A 程在全
%J 遗传
%D 2008
%I 
%X The cDNA library of Yuanjiang Oryza rifupongon leaf was constructed by using SMARTTM technology. The titers of the non-amplified library and the amplified library were 1.1×106 pfu/mL and 3.98×107 pfu/mL, respectively. The recombi-nation rate was more than 91%. The DNA sequence length of the most cDNAs in the library was between 500-2 000 bp. Some cDNAs chosen by random were sequenced. After BLAST analysis of some cDNAs, their possible function were pre-dicted. It is found that these cDNAs show 98% similarity to Oryza sativa japonica in the NCBI database. These provided a base for further study on the structure and function of these cDNAs and evolutionary process of Yuanjiang Oryza rifupongon .
%K cDNA文库
%K 元江普通野生稻
%K 滴度
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=769C9876A5C74239866B5C82E72BC4AB&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=B31275AF3241DB2D&sid=FE17D37BFC90FA6B&eid=5E191A234CD3698F&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=9