%0 Journal Article
%T Isolation of A Novel Peptide That Binds to BLyS from a 12-mer Phage Display Peptide Library<br>BLyS对噬菌体随机12肽库的筛选
%A DING Yan-Li
%A HAN Wei
%A SHEN Qiong
%A LIU Hui
%A YANG Sheng-Li
%A GONG Yi
%A <br>丁艳丽
%A 韩 威
%A 沈 琼
%A 刘 惠
%A 杨胜利
%A 龚 毅
%J 遗传
%D 2006
%I 
%X A 12-mer phage display peptide library was screened for specific binders against B lymphocyte stimulator (BLyS). After 3 rounds of panning, positive phage clones were enriched. ELISAs were used to identify positive phages, and the DNA encoding the positive peptide (RHKIQLRQNIIT) was cloned and expressed as a GST fusion protein in E.coli. After purification, the identity of the fusion protein was confirmed through its specific binding to BLyS by ELISA. We have obtained a peptide that can bind to BLyS and probably act as an antagonistic peptide against the natural BLyS receptor.
%K BLyS<br>噬菌体肽库
%K 小肽
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=D3E22181E1A2C7C3&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=0B39A22176CE99FB&sid=3D9746C06EC12B45&eid=CC0ECB9C52F1B85F&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=13