%0 Journal Article
%T RAP-PCR技术及其应用 RAP-PCR,from Theory to Applications
%A 李智
%A 李强
%A 孙春晓
%A 于常海
%A LI Zhi
%A LI Qiang
%A SUN Chun-xiao
%A Albert C.H.Yu
%J 遗传
%D 2001
%I 
%X RAP-PCR是以PCR为基础构建RNA指纹图谱,研究基因差异表达的有效方法.所显示的种系特异性差异可用于对基因的遗传作图;所揭示的组织特异性差异可用于研究特异基因的表达.该法可检测各种情形下RNA群体间的差异.本文简介其基本原理及在基因差异表达研究领域的最新应用。 Abstract:RAP-PCR is a useful method based on PCR to display fingerprint of RNA populations and to study differential gene expression.The strain-specific differences revealed should be useful for genetic mapping of genes.The tissue-spe-cific differences revealed might be useful for studying differential gene expression.In this review,we introduce the theory of RAP-PCR and its recent applications.
%K RAP-PCR
%K 基因差异表达
%K 基因克隆
%K Key
%K words
%K RAP-PCR
%K differential
%K gene
%K expression
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=E8CEDB8960388AD992676499F2F89878&yid=14E7EF987E4155E6&vid=EA389574707BDED3&iid=E158A972A605785F&sid=B9B90065CF5CD7F0&eid=DC2727B6AC017B3F&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=0