%0 Journal Article %T 培养基成分对东北红豆杉细胞生长和紫杉醇产量的影响 The Relation between the Cell Growth, the Volume of Taxus Cuspidate and the Composition of its Cell Culture Medium %A 司徒琳莉 %A 李振山SITU Lin-li %A LI Zhen-shan %J 遗传 %D 2001 %I %X 在东北红豆杉(taxuscuspidate)细胞培养物的基本培养基中加入30mg@L-12.4-D,可以提高细胞培养物的生长量,其相对最高生长量可以达到85.999mgFW/gFW@d;培养基中加入活性炭粉末lg@L-1,可以克服植物细胞生长过程中的褐化问题;适当种类及含量的生长调节剂的加入:6-BA0.5mg@L-1+KT2mg@L-1,可以使植物细胞合成和积累相对较多的紫杉醇(Taxol)。 Abstract:The growth quantity of Taxus Cuspidate's cell culture materials can be increased and the highest quantity will amount to 85.999 mgFW/g·FW·d·if We put 2,4-D (30rog·L-1 )into the basic cuhure medium of it; The difficulty of foxiness during the plants growing process can be overcome if we add acive carbon powder(lgL-1)into the culture medium; The plant cell can synthetize and gather more Taxol comparatively with the addition kf proper and amount of growth regulators:6-BA(0.5mg·L-1) + KT2. %K 紫杉醇 %K 细胞培养物 %K 生长量 %K 活性炭 %K 生长调节剂 %K Key %K words %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=0926B9B750C2FA4722D4885F2F95422A&yid=14E7EF987E4155E6&vid=EA389574707BDED3&iid=E158A972A605785F&sid=86C0C9A759FDA8CA&eid=E3691231514F8E11&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=0