%0 Journal Article
%T 利用RAPD标记构建美洲黑杨×欧美分子标记连锁图谱 RAPD Linkage Mapping in a Populus deltoides×Populus euramericana F1 Family
%A 张新叶
%A 尹佟明
%A 诸葛强
%A 黄敏仁
%A 朱立煌
%A 翟文学
%A 邬荣领
%A 王明庥ZHANG Xin-ye
%A YIN Tong-ming
%A ZHUGE Qiang
%A HUANG Min-ren
%A ZHU Li-huang
%A ZHAI Wen-xue
%A WU Rong-ling
%A WANG Ming-xiu
%J 遗传
%D 2000
%I 
%X 本文利用RAPD标记和美洲黑杨(Populus deltoides)×欧美杨(P.euramericana)的F1群体，构建了美洲黑杨×欧美杨的分子标记连锁图谱。实验过程中对1040个寡核苷酸随机引物进行了重复筛选，共选出127个引物用于作图群体(包括双亲共92个无性系)的随机扩增，这127个引物产生229个多态基因座，其中符合“拟测交”1∶1分离的有214个。利用多点连锁分析，形成19个连锁群及6个三连体和14个连锁对。由19个连锁群构成的图谱含标记129个，总图距为1914.2cM，覆盖杨树基因组约73.62％。标记间的平均间距为14.84cM。本研究获得了中等密度的美洲黑杨×欧美杨的一个连锁框架。 Abstract:A molecular linkage map was constructed for the parents of a P.deltoides × P.euramericana F1 family based on random amplified polymorphic DNA(RAPD)markers.A set of 1040 random oligonucleotide primers were screened and 127 primers were selected to generate RAPD markers within a sample of 90 F1 progenies.A total of 229 segregating loci were identified.Among the 229 loci,15 loci were found distorted from the normal 1∶1 ratio.Using multiple analysis,the 214 markers formed 19 main Linkage groups (including 129 markers)and b triples and 14 pairs.The resulting Linkage map of Populus deltoides × P.euramericana (including 129 markers)spanned 1914.2cM(73.62％ coverage of genome length)wi
%K 分子标记连锁图谱
%K RAPD
%K 美洲黑杨×欧美杨
%K Key
%K words
%K molecular
%K linkage
%K map
%K RAPD
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=820E34113EA22793&yid=9806D0D4EAA9BED3&vid=BC12EA701C895178&iid=E158A972A605785F&sid=79D2EF35F60110C2&eid=527AEE9F3446633A&journal_id=0253-9772&journal_name=遗传&referenced_num=25&reference_num=6