%0 Journal Article
%T A novel polymorphism of IGFBP-3 gene and its relation-ship with several wool traits in Chinese Merino sheep<br>IGFBP-3基因多态性及其与中国美利奴羊部分羊毛性状的关联性分析
%A SHEN Min
%A WANG Wen-Jun
%A YANG Yong-Lin
%A GAN Shang-Quan
%A HE Qi-Hong
%A ZHANG Yong-Sheng
%A WANG Jian-Hua
%A MA Chun-Ping
%A LIU Zheng-Shan
%A LIU Shou-Ren
%A LI Ning
%A <br>沈敏
%A 王文君
%A 杨永林
%A 甘尚权
%A 何其宏
%A 张永胜
%A 王建华
%A 马春萍
%A 刘正山
%A 刘守仁
%A 李宁
%J 遗传
%D 2008
%I 
%X In this study, PCR-SSCP analysis was used to identify genetic variation in IGFBP-3 gene in Chinese Merino and Kazakh sheep. A PCR product of 178 bp corresponding to partial intron1 illustrated three unique binding patterns by SSCP analysis. Frequencies of the genotype AA, AB, BB and allele A, B in Chinese Merino sheep were 0.70, 0.24, 0.06, and 0.82, 0.18 respectively , and they were 0.87, 0.13, 0.00, and 0.93, 0.07 respectively in Kazaka sheep. Sequence analysis revealed a G/T transversion at position 122 of the fragment. This polymorphic locus of IGFBP-3 gene was at Hardy-Weinberg dis-equilibrium (Plt;0.01) in the two breeds. Different genotypes slightly affected several wool traits of Chinese Merino sheep. The individuals of genotype AA, AB, and BB had no significant difference in post-shearing weight and clean wool rate. Sta-ple length (SL) was decreased with the genotype of AA, AB, and BB, and the difference between AA and AB was significant (Plt;0.01). Greasy fleece weight (GFW) and follicle density in individuals of genotype AA was significantly lower than that in individuals of genotype AB (Plt;0.01) and BB (Plt;0.05); Average fiber diameter (AFD) in individuals of genotype AA was significantly higher than that in individuals of genotype AB (Plt;0.01) and BB (Plt;0.05).
%K 中国美利奴羊
%K IGFBP-3基因
%K 多态性
%K 羊毛性状
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=B1B1F6FC0F72643F9679B377C0705C08&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=9CF7A0430CBB2DFD&sid=7CCBDF94263DBB99&eid=2E45C5B3364EA9F3&journal_id=0253-9772&journal_name=遗传&referenced_num=5&reference_num=17