%0 Journal Article
%T Progress on methods for purity assessment of separated chromosome X- or Y-bearing sperm<br>X、Y精子分离纯度评价方法的研究进展
%A GUO Jia-Ming
%A ZHU Hua-Bin
%A WANG Dong
%A ZHANG Lin-Bo
%A HAO Hai-Sheng
%A DU Wei-Hua
%A <br>郭家明
%A 朱化彬
%A 王栋
%A 张林波
%A 郝海生
%A 杜卫华
%J 遗传
%D 2008
%I 
%X In this review of methods for purity assessment of isolated chromosome X- and Y-bearing sperm, we compared the principles, operating procedures, as well as pros and cons for various methods. We conclude that nested PCR of single sperm will become a conventional and popular method with lower costs, and the method will play a very important role in optimizing the X, Y sorting method, if the sensitivity and accuracy of the method can be increased and the testing time decreased, and promote the new progress in other genetic testing techniques on single sperm.
%K 精子
%K 分离精子
%K 纯度评价
%K 单精子巢式PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=4B4328E64CBF3B8A6E8A9FAA5C405C92&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=9CF7A0430CBB2DFD&sid=CA10C709B736BBEA&eid=9C76A7541E963918&journal_id=0253-9772&journal_name=遗传&referenced_num=2&reference_num=30