%0 Journal Article
%T 用超常的复性温度改善小麦RA PD分析的效果 Better RAPD Patterns Obtained by Using High Annealing Temperatures in Wheat
%A 刘红彦
%A 牛永春
%A 钟鸣LIU Hong-yan
%A NIU Yong-chun
%A ZHONG Ming
%J 遗传
%D 2001
%I 
%X 用38～66℃不同复性温度处理,比较了18条随机引物的扩增结果.发现复性温度在40～50℃之间均有数量不等的扩增产物,不同引物的最高复性温度不同,有些引物用60℃以上的复性温度仍有扩增产物.一些在35～38℃的复性温度下非特异性产物较多的引物,通过大幅度提高复性温度,能提高扩增产物的特异性,获得清晰的RAPD带型。 Abstract:To decrease nonspecific products and obtain clear RAPD patterns,18 10- mer random primers were tested at different annealing temperatures.The results indicated that all the amplification can be performed when the annealing temperature is in the range of 40~50℃.There were a few primers with which the amplification was still performed when the annealing temperature is above 60℃.By using high annealing temperatures,some primers which produce more nonspecific product at the annealing temperatures of 35~38℃ could generate reproducible and distinct bands.
%K RAPD
%K 复性温度
%K 特异性
%K Key
%K words
%K RAPD
%K annealing
%K temperature
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=C61272F15FDB78FB78AA8B085AA11E7D&yid=14E7EF987E4155E6&vid=EA389574707BDED3&iid=E158A972A605785F&sid=CF6CB42CFF3D4C4E&eid=CFBDB06850C21CC6&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=0