%0 Journal Article
%T Cloning and the sequence analysis of the fish glutathione transferase Pi gene<br>鱼类谷胱甘肽转移酶基因cDNA克隆及其序列分析
%A SUN Yu-Hua
%A XIE Ping
%A GUO Hai-Tao
%A XIA Wen-Wei
%A <br>孙玉华
%A 谢平
%A 郭海涛
%A 夏文伟
%J 遗传
%D 2007
%I 
%X Using RT-PCR method,the glutathione transferase Pi cDNAs were cloned from Cyprinus carpio,Hy-pophthalmichthys molitrix,and Carassius auratus.The open reading frames(ORFs) from the 3 fishes were 627 bp long(encoding for 208 amino acids) with the initial code ATG and the terminal code TGA.The sequence similarity was 50% between fish and mammals,33% between fish and amphibian,and 15% between fish and arthropoda,respectively.The sequence similarity was big among fishes,and the average value of the 4 cyprinids was about 85%.Phylogenetic tree was constructed for 13 species based on GST Pi amino acid sequences using MP(Maximum Parsimony) method.Two major clusters were recognized:cluster one consisted of Mammals(bootstrap 100) and cluster two consisted of fishes(bootstrap 93) .Based on the sequences analyses of N/C domain of GST Pi,we proposed the detoxification mechanism of freshwater fishes that were thought to have stronger tolerance to microcystins.
%K glutathione transferase Pi
%K Cyprinus carpio
%K Hypophthalmichthys molitrix
%K Carassius auratus<br>谷胱甘肽转移酶Pi型
%K 鲢
%K 鲤
%K 鲫
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=D44287ED4C097DEF&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=38B194292C032A66&sid=31125890FF093250&eid=0954045FA0C6885F&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=15