%0 Journal Article
%T Molecular cloning and characterization of BcMYBogu, a novel member of the MYB family involved in OguCMS in Brassica campestris ssp. chinensis<br>白菜OguCMS相关MYB家族新基因BcMYBogu的克隆与特征分析
%A XIANG Xun
%A CAO Jia-Shu
%A YE Wan-Zhi
%A CUI Hui-Mei
%A YU Jian-Nong
%A <br>向 珣
%A 曹家树
%A 叶纨芝
%A 崔辉梅
%A 俞建浓
%J 遗传
%D 2007
%I 
%X 为研究CMS核质互作的分子机理,将甘蓝型油菜(Brassica napus L.)和白菜(B.campestris L.ssp.chinensis Makino)杂交并连续回交6代获得白菜OguCMS,在与保持系花药细胞学比较的基础上,运用cDNA-AFLP筛选得到白菜OguCMS早、中期花蕾提早表达的MYB-like差异片段,利用RACE克隆得到该片段的cDNA全长,命名为BcMYBogu(GenBank登录号:EF127861),对其氨基酸序列和表达特征进行研究。结果表明,白菜OguCMS绒毡层在四分体后增生,高度液泡化,导致小孢子花粉外壁异常,细胞质同外壁分离并降解;花药变白;BcMYBogu具有典型的MYBDNA结合域—W残基和SHAL]QKYRF]基序;系统进化分析显示BcMYBogu与AtMYB26,AtMYB32和AtMYB4等聚类在同一分枝;RT-PCR分析表明BcMYBogu在莲座叶、花茎和花蕾中均有表达,但在OguCMS花蕾中表达量显著上升。由此推测BcMYBogu是一个新的与白菜OguCMS相关的MYB家族新成员。
%K Chinese cabbage (Brassica campestris ssp
%K chinensis)
%K OguCMS
%K BcMYBogu
%K Tapetum<br>白菜(Brassi
%K cacampestris
%K ssp.chinensis)
%K OguCMS
%K BcMYBogu
%K 绒毡层
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=6B0439BA84FD1CEB&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=94C357A881DFC066&sid=BA3451F2C9E4FB70&eid=3EABEBD973E45554&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=29