%0 Journal Article
%T Applications of SSCP and HMA for polymorphic analysis of horse MHC-I alleles<br>SSCP和HMA方法在马MHC-I类分子基因多态性研究中的应用
%A 项伟
%A 马建
%A 王雪峰
%A 赵玉军
%A 周建华
%J 遗传
%D 2008
%I 
%X Abstract: In this article, we report the analysis of genetic polymorphisms of horse MHC-I molecules by SSCP and HMA, which are methods based on the technique of polyacrylamide gel electrophoresis (PAGE). Our results showed that SSCP was not a suitable method for the analysis of genetic polymorphisms of horse MHC-I molecules due to the failure in gener-ating satisfied separation of DNA fragments, even if experimental conditions were optimized. However, the HMA method produced clearly separated DNA fragments of horse MHC-I molecules, after the experimental conditions, such as the run-ning temperature and the concentration of detergent, were optimized by using a reference plasmid. PCR-amplified samples from horses No. 6, No. 7, No. 8, No. 9 and No. 10 generated 6, 5, 6, 5, and 7 bands, respectively, in corresponding lanes of the polyacrylamide gel. DNA fragments in each band cut from the gel were amplified by PCR using a second pair of prim-ers, and were cloned for sequencing. Alignment analysis of these sequences revealed that HMA was a proper method to efficiently analyze the polymorphisms of MHC-I molecule genes.
%K SSCP
%K HMA
%K 基因多态性
%K MHC-I类分子基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=BE596143DB6056EF00B432A44124ABFA&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=59906B3B2830C2C5&sid=D9BD5E184C6E559D&eid=71706E59E547123D&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=11