%0 Journal Article
%T Cloning of low-molecular-weight glutenin subunit gene from Sichuan wheat landrace AS1643 and its secondary structure prediction<br>四川小麦地方品种AS1643中低分子量谷蛋白基因的克隆及其蛋白质的二级结构预测
%A CHEN Hua-Ping
%A HUANG Qian-Ming
%A WEI Yu-Ming
%A ZHENG You-Liang
%A <br>陈华萍
%A 黄乾明
%A 魏育明
%A 郑有良
%J 遗传
%D 2007
%I 
%X The full-length coding region (open reading frame, ORF) of low-molecular-weight glutenin subunit (LMW-GS ) gene was amplified from Sichuan wheat landrace accession AS1643 by using the primer pairs P1/P2, which were designed according to wheat LMW-GS conservative domains. The amplified DNA fragment was separated and recovered from agarose gel, subsequently cloned into pMD18-T vector, and then transformed into E. coli strain DH5a. One positive clone LMW-AS1643 (GenBank accession No. EF 190322) was selected and sequenced. It had a coding region of 909 bp, and encoded a mature protein of 302 amino acid residues. Sequence analysis suggested that LMW-AS1643 was a typical LMW-GS gene. The deduced amino acid sequence comparison suggested that LMW-AS1643 had a high similarity with other known LMW-GS genes, with the highest similarity of 93.40 %Bioinformatics analysis indicated that among the three types of secondary structures, content of coil was the highest with 67.90 %, a-helix was the second with 30.46%, and b-sheet was the lowest with 1.64%.
%K wheat landrace
%K low-molecular-weight glutenin subunit
%K gene cloning
%K structure prediction<br>小麦地方品种
%K 低分子量谷蛋白基因
%K 基因克隆
%K 结构预测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=CE212FB10410BB9D&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=DF92D298D3FF1E6E&sid=EE7D0B10C851F35D&eid=9C230FD2B3A7F308&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=29