%0 Journal Article
%T Molecular Analysis of THH-Induced Mutations at the HPRT Locus in Human Promyelocytic Leukemia Cells by Multiplex Polymerase Chain Reaction<br>昆明山海棠诱导人白血病细胞HPRT基因突变的研究 Molecular Analysis of THH-Induced Mutations at the HPRT Locus in Human Promyelocytic Leukemia Cells by Multiplex Polymerase Chain Reaction
%A LIU Sheng-xue
%A CAO Jia
%A AN Hui
%A ZHOU Zi-yuan
%A <br>刘胜学
%A 曹佳
%A 安辉
%A 周紫垣LIU Sheng-xue
%A CAO Jia
%A AN Hui
%A ZHOU Zi-yuan
%J 遗传
%D 2000
%I 
%X 为研究昆明山海棠（ＴＨＨ）的遗传毒性和药理作用，采用单细胞克隆培养，双向筛选计数，多重ＰＣＲ扩增与电泳分析，研究了ＴＨＨ诱导ＨＬ－６０细胞ＨＰＲＴ基因突变率及分子突变谱，发现随着染色毒剂量的增加，细胞接种存经逐渐下降，突变频率明显升高；ＴＨＨ诱发突变主要由缺失和点突变两部分组成（４６．６％和５３．４％），而自发突变几乎全是点突变（９２．３％）；ＨＰＲＴ基因突变位点在各个显子的分布较集中于基因的３′
%K HPRT基因
%K 昆明山海棠
%K 基因突变
%K 遗传毒性
%K 抗肿瘤
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=6CF415442C4A1EEA&yid=9806D0D4EAA9BED3&vid=BC12EA701C895178&iid=94C357A881DFC066&sid=407C905D8F0449C4&eid=51C74DF6A16DA45B&journal_id=0253-9772&journal_name=遗传&referenced_num=3&reference_num=6