%0 Journal Article
%T Development and forensic application of a pentaplex X-STR loci typing system<br>5个X-STR基因座荧光复合扩增体系的建立及法医学应用
%A LIU Qiu-Ling
%A LV De-Jian
%A SUN Hong-Yu
%A LU Hui-Ling
%A WU Xiang-Lin
%A WU Xin-Yao
%A <br>刘秋玲
%A 吕德坚
%A 孙宏钰
%A 陆惠玲
%A 伍祥林
%A 伍新尧
%J 遗传
%D 2007
%I 
%X A fluorescent multiplex PCR that simultaneously amplifies five X-chromosomal short tandem repeat(X-STR) loci(DXS6803,DXS981,DXS6809,DXS6789 and DXS7132)was set up to study their polymorphic nature and to determine its use in kinship tests for forensic cases.PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer with GeneMapper ID 3.1 Analysis Software.The pentaplex system gave satisfactory results as its sensi-tivity,reproducibility and unambiguous genotyping.About 20 ng DNA was routinely used,although 0.25 ng DNA was suf-ficient for allele typing.The results demonstrate that the multiplex system of the five X-STR loci provides a fast technology for forensic identification and paternity testing.The X-STR pentaplex system can complement the analysis of AS-STR and Y-STR efficiently,especially in complex cases of kinship testing.
%K X-STR
%K fluorescent multiplex PCR
%K kinship testing
%K forensic application<br>X染色体短串联重复(X-STR)
%K 荧光复合扩增
%K 亲权鉴定
%K 法医学应用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=35273B7C85B2E5497831EDEE5EF1FFBF&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=59906B3B2830C2C5&sid=AA170179913DEBCD&eid=B60306E6036A044A&journal_id=0253-9772&journal_name=遗传&referenced_num=2&reference_num=11