%0 Journal Article
%T Constructing the recombinant of pET28a-TAT-LacZ<br>pET28a-TAT-LacZ重组子的构建
%A Shi-Rong Yan
%A Jian Gong
%A Jie Yan
%A Yun-Cheng Qiu
%A <br>严世荣
%A 龚坚
%A 严洁
%A 邱云城
%J 遗传
%D 2003
%I 
%X A synthesized double-stranded oligomeric nucleotide encoding 11-amino acid TAT protein transduction domain3 was inserted into pET28a vector after 6 histidine coding sequence, LacZ gene from pcDNA4/Myc-His/LacZ was digested by EcoR I and Hind III, then cloned into pET28a-TAT. The highly expressed TAT-beta-galactosidase was purified by affinity chromatography. TAT-beta-Gal fusion protein can across vascular smooth muscle cells in vitro. This result open new possibility for direct delivery of protein into tissues for therapy.
%K pET28a质粒
%K TAT蛋白转导区
%K TAT－β－Gal融合蛋白
%K 平滑肌细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=84251E2214F2F7B9&yid=D43C4A19B2EE3C0A&vid=C5154311167311FE&iid=0B39A22176CE99FB&sid=A8DE7703CC9E390F&eid=3986B25773CB6C30&journal_id=0253-9772&journal_name=遗传&referenced_num=4&reference_num=5