%0 Journal Article
%T Polymorphism Analysis of the Goat Growth Hormone Gene in the 5'';Regulatory Sequence<br>山羊生长激素基因5''调控区的多态性分析
%A LI Mei-Yu~
%A MIN Ling-Jiang~
%A SUN Guo-Qiang~
%A PAN Qing-Jie~
%A SHEN Wei~
%A WANG Guang-Lian~
%A <br>李美玉
%A 闵令江
%A 孙国强
%A 潘庆杰
%A 沈伟
%A 王广莲
%J 遗传
%D 2004
%I 
%X The polymorphism of goat growth hormone gene in 5'-region in 274 goats including Lubei white goat, introducted Boer goat, pure bred Boer goat , hybrid generation 1 of LuBei white goat and Boer goat , backcross of hybrid generation 1 and Boer goat were detected by PCR-SSCP with two different pairs of primers. The result showed that in 26-239bp most of the Boer goats and hybrid generation presented as AA genotype,while most LuBei white goats presented as BB genotype. In 225-429bp all breeds presented as more CC genotype.The amplified fragments of AA,BB and CC,DD of the two fragments were cloned and sequenced . The result showed that in 26-239bp AA genotype had one substitution mutation at 60 site (C-->T), and base C losing at 211 site and DD genotype had three substitution mutations at 264 site (T-->C), 292 site (T-->A) and 372 site (C-->T) in 225-429bp. The results above-mentioned first confirmed that there were polymorphisms in 5' region of GH gene.
%K goat
%K growth hormone gene
%K PCR-SSCP
%K genetic polymorphism<br>山羊
%K 生长激素基因
%K PCR—SSCP
%K 遗传多态性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=3967EBC1C0B7A426&yid=D0E58B75BFD8E51C&vid=96C778EE049EE47D&iid=B31275AF3241DB2D&sid=6D80B994DAB4686B&eid=9EB9AF946ABE60ED&journal_id=0253-9772&journal_name=遗传&referenced_num=13&reference_num=11