%0 Journal Article
%T Isolation of New Zinc Finger Genes through cDNA Library Screening Combined with RACE<br>用RACE结合cDNA文库筛选的方法获取新的锌指蛋白基因 Isolation of New Zinc Finger Genes through cDNA Library Screening Combined with RACE
%A DU Zhan wen
%A LIU Li ren
%A ZHANG Jun wu
%A <br>杜占文
%A 刘立仁
%A 张俊武
%J 遗传
%D 2002
%I 
%X Most of the important functionally proteins contain the corresponding function domains that consist of conserved amino acid sequences.The study provided a method to identify novel genes that encode proteins containing important functionally domains with conserved sequences.First,primers were designed according to the sequence of the cDNA library vector and the ESTs that have been obtained by reverse PCR and degenerate primers encoding Zinc finger domain.The cDNA library DNA was used as template for PCR amplification.The amplified fragment that contains nonhomologous sequences of the cDNA was inserted into pGEM-T easy vector.The fragment was recovered and used as a probe for screening the cDNA library.Several cDNAs with full length that encode proteins with Zinc finger domain and represent the original ESTs have been successfully cloned from a human bone marrow cDNA library.This strategy can also be used in screening genes that encode proteins containing differential function domains with conserved sequences.
%K gene cloning
%K rapid amplification of cDNA end (RACE)
%K nonhomologous DNA sequences
%K cDNA library screening<br>RACE
%K cDNA文库
%K 筛选
%K 锌指蛋白基因
%K 非同源DNA序列
%K 新基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=3A67265DFC20BDE7&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=C6EC7357BCACD3A4&eid=717CC18E05F2AFA0&journal_id=0253-9772&journal_name=遗传&referenced_num=6&reference_num=3