%0 Journal Article %T Application of Laser Capture Microdissection in Plant Genomic Research
激光捕获显微切割技术在植物基因组研究中的应用 %A CAI Min-Hua %A HU Ying-Kao %A LI Ya-Xuan %A YAN Yue-Ming %A
蔡民华 %A 胡英考 %A 李雅轩 %A 晏月明 %J 遗传 %D 2006 %I %X To a large degree, the growth and development of plants depend on the organ and/or cell type-specific expression of genes. However, it can be difficult to obtain sufficient number of specific cells from any developmental stage to analyze expression of genes. Laser capture microdissection (LCM) is a novel technique that allows us to collect pure targeted cell subgroup or even a single cell quickly and precisely, thus the problem of tissue heterogeneity in molecular analysis can be resolved successfully. In this paper, the principles of LCM were introduced and the application of LCM in plant was summarized in gene expression analysis, proteomics and plant-microbe interaction. Meanwhile, possible directions of LCM in plant were put forward. %K laser capture microdissection (LCM) %K gene expression analysis %K proteomics %K plant-microbe interaction
激光捕获显微切割 %K 基因表达分析 %K 蛋白组 %K 植物-微生物互作 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=192E7F01BEEC2A56&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=F3090AE9B60B7ED1&sid=E84660E787B699A9&eid=F4C7ED9FD9D7FC69&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=22