%0 Journal Article
%T Molecular Cloning and Homologous Modeling of Wulong Goose MHC ClassⅠGene<br>五龙鹅MHC ClassⅠ基因克隆及同源建模研究
%A JIA Xiao-Hui
%A WANG Bao-Wei
%A WANG Lei
%A LI Zhen
%A ZHANG Ming-Ai
%A WU Xiao-Ping
%A LIU Guang-Lei
%A YANG Zhi-Gang
%A LONG Fang-Yu
%A ZHANG Xu-Hui
%A <br>贾晓晖
%A 王宝维
%A 王雷
%A 李桢
%A 张名爱
%A 吴晓平
%A 刘光磊
%A 杨志刚
%A 龙芳羽
%A 张旭晖
%J 遗传
%D 2006
%I 
%X Major histocompatibility complex (MHC) was correlated with immune response for extra-antigen. MHC sequences of chicken, other birds, crawl species and mammalian were derived from GenBank/DDBJ/EMBL and analyzed by alignment, and then primers were designed. By means of LA-PCR method, MHC Classgene was cloned from Wulong goose genomic DNA and total RNA, and the structure of MHC Classgenomic DNA was analyzed using bioinformation methods. The results showed that the genomic DNA, which consisted of 8 extrons and 7 introns, had 64.1% amino acids homologous to chicken and 42.9% amino acids to human. The molecular phylogenetic tree further revealed evolutionary relationship between Wulong goose and other animals such as chicken, other birds, crawl species, mammalian and human. The results of homologous modeling showed that Wulong Goose MHC Classgene was formed by amidocyanogen (N) terminal structure region and carboxyl (C) terminal structure region.
%K MHC
%K ClassⅠ
%K 五龙鹅
%K 基因组
%K 分子进化树
%K 氨基酸同源率
%K 同源建模
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=EC061B8D56CBC307&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=9CF7A0430CBB2DFD&sid=DE3C727FE2D9509D&eid=8AF49F1519D8BF1E&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=17