%0 Journal Article
%T Genetic linkage map of Betula pendula Roth and Betula platyphylla Suk based on random amplified polymorphisms DNA markers<br>白桦RAPD遗传连锁图谱的构建
%A JIANG Ting-Bo
%A LI Shao-Chen
%A GAO Fu-Ling
%A DING Bao-Jian
%A QU Yue-Jun
%A TANG Xin-Hua
%A LIU Gui-Feng
%A JIANG Jing
%A YANG Chuan-Ping
%A <br>姜廷波
%A 李绍臣
%A 高福铃
%A 丁宝建
%A 曲跃军
%A 唐鑫华
%A 刘桂丰
%A 姜静
%A 杨传平
%J 遗传
%D 2007
%I 
%X Based on the genetic inheritance and segregation of random amplified polymorphism DNA (RAPDs) markers, the first middensity linkage map for silver birch was constructed by using a pseudotestcross mapping strategy. A segregat- ing population including 80 progenies from the cross between Betula pendula Roth and B. platyphylla Suk was obtained. A set of 1,200 random oligonucleotide primers were screened, and 208 primers were selected to generate RAPD markers within a sample of 80 F1 progenies. A total of 364 segregating sites were identified. Among them, 307 belonged to 1 : 1 segregating site, and 36 belonged to 3 : 1 segregating site, others were found distorted from the normal 1 : 1 ratio. Alto- gether 307 sites segregating 1 : 1 (testcross configuration) were used to construct parent-specific linkage maps, 145 for B. pendula and 162 for B. platyphylla. The resulting linkage maps consisted of 145 marker sites in 14 groups (four or more sites per group), 6 triples and 6 pairs for B. pendula, which covered the map distance about 955.6 cM (Kosambi units). The average map distance between adjacent markers was 14.9 cM, and 162 linked marker site for B. platyphylla were mapped onto 15 groups (four or more sites per group), 4 triples and 6 pairs, which covered the map distance about 1,545.8 cM, and the average map distance between adjacent markers was 15.2 cM. Further study is warranted to intergrate the two maps toone density map and to locate important genes on the maps.
%K Molecular marker
%K linkage map
%K random amplified polymorphic DNA
%K Betula pendula Roth
%K Betula platyphylla Suk
%K pseudo-testcross<br>分子标记
%K 连锁图谱
%K 随机扩增多态性DNA
%K 欧洲白桦:中国白桦
%K 拟测交
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=EF4087DA4EC7775F&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=DF92D298D3FF1E6E&sid=65A51D0EBEB846F5&eid=BF1420E7E18952EE&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=18