%0 Journal Article
%T Generation of bone morphogenetic protein 4 conditional RNA inter-ference mice<br>骨形成蛋白4条件性RNA干扰小鼠的建立
%A LI Fu-Bing
%A DU Xiao-Lan
%A YU Ying
%A ZHAO Ling
%A HE Qi-Fen
%A CHEN Lin
%A <br>李福兵
%A 杜晓兰
%A 余瑛
%A 赵玲
%A 何启芬
%A 陈林
%J 遗传
%D 2008
%I 
%X To explore bone morphogenetic protein 4 (BMP4) function in the developing bone, a BMP4 conditional RNA interference (CRNAi) vector was constructed based on the pBSK/U6 vector with a LoxPneo cassette. The transgene fragment targeting bmp4 was obtained by Kpn and Afl double digestion and was purified before being microinjected into fertilized eggs from FVB/NJ mice. BMP4CRNAi transgenic mice were genotyped by PCR. And the PCR positive mice were crossed with Col2a1-Cre transgenic mice, whose Cre recombinase was specifically expressed in osteo-chondro-progenitor cells. Bmp4 mRNA expression in primary chondrocytes were examined by semi-quantitive RT-PCR to determine RNA interference efficiency. Results showed that BMP4(CRNAi) mice and BMP4 (Col2a1-CRNAi) mice were produced successfully, and bmp4 knockdown efficiency in primary chondrocytes of BMP4 Col2a1-CRNAi mice was 81%. This transgenic mouse line provides excellent model for studying the role of BMP4 in chondrocyte development, and BMP4CRNAi mouse may be a good model for studying the role of BMP4 in different cells, tissues and organs through crossing with different Cre transgenic mice.
%K 骨形成蛋白4
%K 条件性RNA干扰
%K 条件性敲低
%K 转基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=7BD938E0E80E236E7B3D621590E1E2B0&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=38B194292C032A66&sid=4B168891B5E5FB30&eid=C7DDDE86E6286CD9&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=18