%0 Journal Article
%T Progress on Gene Targeting<br>基因打靶技术的研究进展
%A Hong-quan Liu
%A Ji-xun Dai
%A Wen-gong Yu
%A Kun-feng Yang
%A <br>刘红全
%A 戴继勋
%A 于文功
%A 杨堃峰
%J 遗传
%D 2002
%I 
%X Gene targeting is a rising technology in molecular biology,which is defined as the introduction of exogeneous DNA to specific site in genome by homologous recombination,and consequently change the hereditary character of the cell.This technology provides a new and powerful means for research in developmental biology,molecular genetics,immunology and medicine.Progresses have been made in exploring gene structure and function,gene expression and regulation,transgene and gene therapy with the application of gene targeting.But there are some problems in gene targeting,especially for the low efficiency.This article just provided a review of the principle and program of gene targeting,and discussed the possible approaches to increase the efficiency of gene targeting.
%K gene targeting
%K homologous recombination
%K targeting vector
%K targeting efficiency<br>基因打靶技术
%K 研究进展
%K 同源重组
%K 打靶载体
%K 打靶效率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=7A771E4AF74BF6E7&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=B31275AF3241DB2D&sid=E008F9AD6D4B96EF&eid=FD6137FFCE59D193&journal_id=0253-9772&journal_name=遗传&referenced_num=11&reference_num=48