%0 Journal Article
%T 氯化高铁血红素诱导K562细胞中β-珠蛋白基因表达的研究 A Study of Inductive Effect of Hemin on Expression of theβ-globin Genes in K562 Cells
%A 贾春平
%A 陈美珏
%A 黄淑帧
%A 曾溢滔JIA Chun-ping
%A CHEN Mei-jue
%A HUANG Shu-zhen
%A ZENG Yi-tao
%J 遗传
%D 2002
%I 
%X 以绿色荧光蛋白（EGFP）基因为报道基因，构建了在β-珠蛋白启动子驱动及HS2元件调控下的重组表达载体HG，用脂质体转染法将其转染到K562细胞中，并用RT-PCR方法及流式细胞仪检测氯化高铁血红素(Hm)对K562细胞中β-珠蛋白基因表达及重组载体HG在K562细胞中瞬时表达的影响。结果显示：用30μmol/L Hm诱导K562细胞24、48及72h后，不仅其γ-珠蛋白mRNA水平升高，其β-珠蛋白mRNA水平也明显上升，而且这种诱导作用在诱导24、48h后比较明显；Hm还可增强重组表达载体HG在K562细胞中的瞬时表达。提示Hm诱导红系分化的机理可能与γ→β-珠蛋白基因的转换机制相关。 Abstract: The recombinant plasmid HG was constructed,in which the reporter gene encoding the enhanced green fluorescent protein (EGFP) was driven by the β-globin promoter and regulated under the HS2 element.The inductive effect of hemin on the expression of the β-globin gene and transiently transfected β-globin genes in K562 cells was analysed by FACS as well as RT-PCR method.The results showed that the level of γ and β-globin gene mRNA in K562 cells increased significantly after 24,48 and 72 hours induced with 30 μmol/LHm.And this inductive effect was sronger after 24 and 48 hours.Furthermore,the transient expression of plasmid HG in K562 cells increased significantly with hemin induction.Th
%K 氯化高铁血红素
%K K562细胞
%K γ-珠蛋白基因
%K β-珠蛋白基因
%K Key
%K words
%K emin
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=EED13D136364B972304A922FAF1A3C2F&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=E158A972A605785F&sid=87545994EC2C1F12&eid=F1A8654ADB4E656E&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=0