%0 Journal Article
%T Compare of Full-length cDNA Library Construction Methods<br>几种全长cDNA文库构建方法比较
%A MAO Xin-Guo
%A JING Rui-Lian
%A KONG Xiu-Ying
%A ZHAO Guang-Yao
%A JIA Ji-Zeng
%A <br>毛新国
%A 景蕊莲
%A 孔秀英
%A 赵光耀
%A 贾继增
%J 遗传
%D 2006
%I 
%X The use of full-length cDNA libraries is an effective tool to obtain complete gene information in a high-efficiency, high-throughput manner, especially in organisms with huge genomes that are not amenable to whole genome sequencing. In this review, we outlined several methods of full-length cDNA library construction and compared their advantages and disadvantages based on their respective principles. Drawing on our own experience, we described the Cap-trapper method in detail, with an emphasis on its application in wheat full-length cDNA library construction as well as the determination of the ratio of full-length cDNA in a library.
%K Full-length cDNA library
%K Cap-trapper method
%K Capture method
%K Oligo-capping method
%K Cap-jumping method
%K SMART method<br>全长cDNA文库
%K Cap-trapper法
%K Capture法
%K Oligo-capping法
%K Cap-jumping法
%K SMART法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=0156F0B06E1A73AD&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=DF92D298D3FF1E6E&sid=461E94ABCF58C63F&eid=BF1420E7E18952EE&journal_id=0253-9772&journal_name=遗传&referenced_num=20&reference_num=29