%0 Journal Article
%T Developmental expression changes of insulin-like growth factors (IGFs) system genes in longissimus dorsi muscle of two pig breeds<br>猪背最长肌中胰岛素样生长因子(IGFs)系统基因的发育表达模式
%A GU Yi-Ren
%A ZHANG Kai
%A LI Ming-Zhou
%A LI Xue-Wei
%A ZHU Li
%A WANG Jin-Yong
%A CHEN Lei
%A <br>顾以韧
%A 张凯
%A 李明洲
%A 李学伟
%A 朱砺
%A 王金勇
%A 陈磊
%J 遗传
%D 2009
%I 
%X Real-time fluorescence quantitative PCR was applied to investigate the developmental expression patterns of insulin like growth factor-1 and -2 (IGF-1 and -2), insulin like growth factor receptor -1 and -2 (IGF-1R and -2R), insulin like growth factor binding protein-3 and -5 (IGFBP-3 and -5) genes at the age of birth (0), 1, 2, 3, 4, and 5 months in long-issimus dorsi muscle of Landrace and Meishan pigs. The expression level of IGF-1 exhibited up-regulation, in contrast, the expression level of IGF-2 showed down-regulation after birth in the two pig breeds. These findings are consistent with the previous knowledge that IGF-2 is mainly expressed during the embryonic phase, IGF-1 is mainly expressed during the postnatal period, which could promote cell proliferation and ontogenesis. The expression patterns of IGFRs and IGFs were dissimilar from 0 to 5 months in the two pig breeds, which suggest that the expression changes of IGFRs may not be sub-jected to the regulation by local secretion of IGFs. Strikingly, at the age of 2 months, the expression levels of IGF-1R, IGF-2R, and IGFBP-3 reached their maximum peak values in Landrace pigs, which suggest that the 2 months after birth may be the most important growth stage for genes of insulin-like growth factors (IGFs) system to exert their biological functions from birth to 5 months. These results indicate that the mRNAs of IGFs system genes exhibit specific develop-mental expression changes between Landrace and Meishan pigs, and provides some data for further study on the mutual regulation mechanism of genes in IGFs system.
%K 猪
%K 背最长肌
%K 荧光实时定量PCR
%K 胰岛素样生长因子
%K 胰岛素样生长因子受体
%K 胰岛素样生长因子结合蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=A3E5B1530EC9338878D1EE0CDB84D5A9&yid=DE12191FBD62783C&vid=4AD960B5AD2D111A&iid=5D311CA918CA9A03&sid=2C8B50BA95995EA2&eid=85C7135C065B9251&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=30