%0 Journal Article
%T Effects of Carbon Sources on the Expression of cry1D -lacZ in Bacillus thuringiensis<br>碳源对苏云金芽胞杆菌融合基因cry1D-lacZ表达的影响 ffects of Carbon Sources on the Expression of cry1D -lacZ in Bacillus thuringiensis
%A Aronson IArthur
%A <br>张利莉
%A 喻子牛
%J 遗传
%D 2002
%I 
%X In order to investigate the influence of the carbon source on the expression of cry1D -lacZ in Bacillus thuringiensis with different genetic backgrounds, cry1D -lacZ was introduced into different strains and cultured in liquid medium containing different carbon sources. Analysis of activity of beta-galactosidase showed that cry1D -lacZ expressed differently in the same host because of different carbon sources and differently in different hosts utilizing same carbon sources,suggesting that metabolism pathway of carbon source in HD-133-5 was special. Sodium succinate as carbon source could lead to earlier sporulation and earlier expression of cry1D -lacZ. When sodium acetate acted as carbon source, cry1D -lacZ could keep high, stable expression in different hosts. These results are helpful for future fermentation of Bacillus thuringiensis.
%K Bacillus thuringiensis
%K gene expression
%K carbon sources<br>碳源
%K 苏云金芽胞杆菌
%K 融合基因
%K cry1D－lacZ
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=AEA76D34077C96E4&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=DFEE4E8C33C95CEF&eid=8ED630AD8C61FAE8&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=15