%0 Journal Article
%T PC-1 enhances c-myc gene expression in prostate cancer cells<br>PC-1在前列腺癌细胞中促进c-myc基因的表达
%A YU Lan
%A SHI Qing-Guo
%A QIAN Xiao-Long
%A LI Shan-Hu
%A WANG Hong-Tao
%A WANG Le-Le
%A ZHOU Jian-Guang
%A <br>俞岚
%A 施庆国
%A 钱晓龙
%A 李山虎
%A 王洪涛
%A 王乐乐
%A 周建光
%J 遗传
%D 2010
%I 
%X PC-1(Prostate and colon gene 1) gene belongs to TPD52 (Tumor Protein D52) gene family. The expression of PC-1 is found to promote androgen-independent progression. This study was conducted to assess the mechnism of promotion of androgen-independent progression in PC-1 gene. The c-myc gene expression was tested by RT-PCR and Western blotting analyses in the LNCaP-pc-1 and LNCaP-zero cell line. After separation of cytoplasm and nulear proteins of the LNCaP-pc-1 and LNCaP-zero cell line, the β-catenin protein was detected by Western blotting. C4-2 cell line was used to examine the effects of 10058-F4 on the PC-1 gene expression. The results of RT-PCR and Western blotting indicated that PC-1 enhanced c-myc gene expression in prostate cancer cells, PC-1 was also found to enhance β-catenin expression in nuclear. Furthermore, a small-molecule c-Myc inhibitor, 10058-F4 represses PC-1 gene expression in C4-2 cell line. Our findings suggest that PC-1 enhances c-myc gene expression in prostate cancer cells through the Wnt/β-catenin pathway. Meanwhile, c-myc plays a feed-forward role in enhancing PC-1 driven c-myc gene expression, and promotes prostate an-drogen-independent progression.
%K 前列腺癌
%K 前列腺癌相关基因(PC-1)
%K c-myc基因I&beta
%K -catenin蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=38CF66E73126053CEFBB0FBD68043549&yid=140ECF96957D60B2&vid=9971A5E270697F23&iid=E158A972A605785F&sid=375BEEEA164CFE59&eid=EA64BD0B2FF6E786&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=14