%0 Journal Article
%T Effect of B-RAF-specific RNA interference on gastric cancer BGC823 cell line<br>B-RAF基因特异的siRNA干扰对胃癌BGC823细胞的影响
%A FU Hao
%A ZHAO Dan-Yi
%A SUN Xiu-Ju
%A HUA Jun
%A YAN Yang
%A QIU Guang-Rong
%A SHANG Chao
%A FU Wei-Neng
%A SUN Kai-Lai
%A <br>付浩
%A 赵丹懿
%A 孙秀菊
%A 滑君
%A 阎杨
%A 邱广蓉
%A 尚超
%A 富伟能
%A 孙开来
%J 遗传
%D 2007
%I 
%X To investigate the influence of B-RAF-specific RNA interference on the proliferation and apoptosis of gastric cancer BGC823 cell line. B-RAF-siRNA and scramble-siRNA were synthesized and transfected into BGC823 cells by TransMessenger. The expression of B-RAF gene and Bcl-2 gene in BGC823 cells was detected by RT-PCR and the level of apoptosis was evaluated in transfected cells by flow cytometry. Results showed that TransMessenger could effectively transfect B-RAF-siRNA and scramble-siRNA into BGC823 cells. B-RAF-siRNA significantly inhibited the expression of B-RAF gene and Bcl-2 gene in BGC823 cells by more than 90% to 100%. B-RAF-siRNA inhibited BGC823 cell prolifera- tion and induced apoptosis (P < 0.01). In conclusion, B-RAF-siRNA can effectively inhibit the expression of B-RAF gene and Bcl-2 gene, induce cell apoptosis and inhibit the proliferation of gastric cancer BGC823 cells.
%K RNA interference
%K B-RAF gene
%K gastric cancer
%K cell apoptosis<br>RNA干扰
%K B-RAF基因
%K 胃癌细胞
%K 细胞凋亡
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=692112068D0D4FCA&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=94C357A881DFC066&sid=FD207D3C5E9776FA&eid=DFBC046213B3DD86&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=17