%0 Journal Article
%T Rapid Accumulation of Polymorphonuclear Neutrophils in the Corpus luteum during Prostaglandin F2¦Á-Induced Luteolysis in the Cow
%A Koumei Shirasuna
%A Sineenard Jiemtaweeboon
%A Sybille Raddatz
%A Akane Nitta
%A Hans-Joachim Schuberth
%A Heinrich Bollwein
%A Takashi Shimizu
%A Akio Miyamoto
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0029054
%X Prostaglandin F2¦Á (PGF2¦Á) induces luteolysis within a few days in cows, and immune cells increase in number in the regressing corpus luteum (CL), implying that luteolysis is an inflammatory-like immune response. We investigated the rapid change in polymorphonuclear neutrophil (PMN) numbers in response to PGF2¦Á administration as the first cells recruited to inflammatory sites, together with mRNA of interleukin-8 (IL-8: neutrophil chemoattractant) and P-selectin (leukocyte adhesion molecule) in the bovine CL. CLs were collected by ovariectomy at various times after PGF2¦Á injection. The number of PMNs was increased at 5 min after PGF2¦Á administration, whereas IL-8 and P-selectin mRNA increased at 30 min and 2 h, respectively. PGF2¦Á directly stimulated P-selectin protein expression at 5¨C30 min in luteal endothelial cells (LECs). Moreover, PGF2¦Á enhanced PMN adhesion to LECs, and this enhancement by PGF2¦Á was inhibited by anti-P-selectin antibody, suggesting that P-selectin expression by PGF2¦Á is crucial in PMN migration. In conclusion, PGF2¦Á rapidly induces the accumulation of PMNs into the bovine CL at 5 min and enhances PMN adhesion via P-selectin expression in LECs. It is suggested that luteolytic cascade by PGF2¦Á may involve an acute inflammatory-like response due to rapidly infiltrated PMNs.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0029054